Protein A column, followed by peptide affinity purification
免疫原
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S149 of human MAP2K5.
MAP2K5
适用: 人, 大鼠
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), FACS
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
抗原
MAP2K5
(Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))
MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MEK5, MKK5