(2 references)-
- 抗原 See all IL1R1 抗体
- IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL1R1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- IL-1 receptors from the C57BL/6 mouse T lymphoma EL4.IL2
- 克隆位点
- 35F5
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IL1R1 Primary Antibody
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anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 226-318) antibody
IL1R1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 21-109) antibodyIL1R1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 119-217) antibodyIL1R1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 481-569) antibodyIL1R1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 386-553) antibodyIL1R1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 18-167) antibodyIL1R1 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4D2D12 unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (pTyr496) antibodyIL1R1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 226-352) antibodyIL1R1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 474-503) antibodyIL1R1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1 Receptor, Type I (IL1R1) (AA 18-320) antibodyIL1R1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow cytometry: Since this antigen may be expressed at low density on the cell surface, investigators may wish to perform staining using Biotin Goat Anti-Rat Ig (Cat. No. 554014) coupled with a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061).
Western Blot: The 35F5 antibody has been reported to be useful for western blotting of non-reduced proteins and being non-reactive to reduced proteins. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." in: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, (1991) (PubMed).
: "Two high-affinity interleukin 1 receptors represent separate gene products." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 8029-33, (1989) (PubMed).
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: "Inhibition of interleukin 1 (IL-1) binding and bioactivity in vitro and modulation of acute inflammation in vivo by IL-1 receptor antagonist and anti-IL-1 receptor monoclonal antibody." in: The Journal of experimental medicine, Vol. 173, Issue 4, pp. 931-9, (1991) (PubMed).
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- 抗原
- IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
- 别名
- CD121a (IL1R1 产品)
- 别名
- CD121A antibody, D2S1473 antibody, IL-1R-alpha antibody, IL1R antibody, IL1RA antibody, P80 antibody, CD121a antibody, CD121b antibody, IL-iR antibody, Il1r-1 antibody, DER4 antibody, Fit-1 antibody, Ly84 antibody, ST2L antibody, St2 antibody, St2-rs1 antibody, T1 antibody, T1/ST2 antibody, interleukin 1 receptor type 1 antibody, interleukin 1 receptor, type I antibody, interleukin 1 receptor-like 1 antibody, IL1R1 antibody, Il1r1 antibody, Il1rl1 antibody
- 背景
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The 35F5 antibody reacts with the type-I interleukin (IL-1) receptor (CD121a) expressed by T-cell, fibroblast, and epithelial cell lines such as EL-4 and 3T3. Expression of the 35F5 epitope on normal mouse thymocytes and splenocytes is undetectable by flow cytometry. CD121a is an 80-kDa type-I transmembrane glycoprotein, a member of the Ig superfamily which mediates the inflammatory responses of leukocytes to IL-1. mAb 35F5 is capable of blocking the binding of IL-1alpha, IL-1beta, and IL-1 receptor antagonist to the type-I IL-1 receptor. The 35F5 mAb is highly specific for the type-I IL-1 receptor and does not react with the type-II IL-1 receptor (CD121b) which is present on murine macrophage and B-cell lines and polymorphonuclear leukocytes (PMN) and PMN progenitors.
Synonyms: IL-1 Receptor, Type I/p80 - 途径
- NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints
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