TNFRSF1B 抗体
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- 抗原 See all TNFRSF1B 抗体
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TNFRSF1B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- Partially purified preparations of TNF binding proteins from U937 cells.
- 克隆位点
- Utr1
- 亚型
- IgG1
- Top Product
- Discover our top product TNFRSF1B Primary Antibody
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anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) antibody
TNFRSF1B 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 2H11C2 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 23-257) antibodyTNFRSF1B 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 7G8B6 unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (C-Term) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (N-Term) antibodyTNFRSF1B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 30-250) antibodyTNFRSF1B 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (C-Term) antibodyTNFRSF1B 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) (AA 1-461) antibodyTNFRSF1B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人 WB, ELISA, IHC, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) antibodyTNFRSF1B 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Immunohistology: 2-5 μg/mL on Cryostat Sections (2 % Periodate-lysine-paraformaldehydeor acetone for fixation). Flow Cytometry: 10 μg/mL. Immunoprecipitation. Western blots: 30 μg/mL on non-reduced SDS PAGE, the epitope is sensitive to reduction,may need enrichment by Immunoprecipitation. Functional Assay: This antibody utr1 is useful for studying biological effects ofTNF-Receptor p75 in vitro where it inhibits the binding of radiolabelled TNF to human cellsexpressing the p75 TNF receptor. In order to completely block TNF binding to the cellsurface, 10 μg/mL of utr1 are required. utr1 itself may have an agonistic effect in assaysmeasuring cytotoxicity, fibroblast growth or IL-6 secretion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- 限制
- 仅限研究用
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- 浓度
- 2.0 mg/mL (by Absorbanece at 280 nm)
- 缓冲液
- PBS, pH 7.2, None, None
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C/-80 °C
- 储存方法
- Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.
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- 抗原
- TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
- 别名
- CD120b / TNFR2 (TNFRSF1B 产品)
- 别名
- CD120b antibody, TBPII antibody, TNF-R-II antibody, TNF-R75 antibody, TNFBR antibody, TNFR1B antibody, TNFR2 antibody, TNFR80 antibody, p75 antibody, p75TNFR antibody, TNF-RII/TNFRSF1B antibody, TNFR-II antibody, TNFRSF1B antibody, TNF-R2 antibody, TNF-alphaR2 antibody, TNFRII antibody, TNFalpha-R2 antibody, Tnfr-1 antibody, Tnfr2 antibody, rank antibody, zgc:163064 antibody, TNF receptor superfamily member 1B antibody, tumor necrosis factor receptor superfamily, member 1b antibody, tumor necrosis factor receptor superfamily, member 1B antibody, TNFRSF1B antibody, Tnfrsf1b antibody, tnfrsf1b antibody
- 背景
- Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor
- 基因ID
- 9606
- UniProt
- P20333
- 途径
- NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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