This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
纯化方法
Affinity Chromatography on Protein G
内毒素水平
Low Endotoxin
免疫原
Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Western Blot. Immunoprecipitation. Radioimmunoassays. Functional Assays. Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Resin Sections. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Requires pre-treatment prior to staining.
实验流程
1. Enzyme pre-treatment using Proteinase K (recommended for tissues fixed for 24 hoursin neutral buffered formalin, NBF): ReagentsA. TE buffer (50 mM Tris base, 1 mM EDTA, pH 8. 0)Tris Base, 6. 10 gEDTA, 0. 37 gDistilled water, 1000 ml
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, None, None
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480