(3 references)-
- 抗原 See all F4/80 (EMR1) 抗体
- F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This F4/80 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应 （详细）
- Cross reactivity: Mouse granulocytes : No, Mouse mast cells : No, Mouse platelets : No, Mouse lymphocytes : No, Mouse fibroblasts : No, Mouse endothelial cells : No
- 过滤
- 0.2 μm filtered
- 克隆位点
- BM8
- 亚型
- IgG2a
- Top Product
- Discover our top product EMR1 Primary Antibody
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anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 732-747) antibody
EMR1 适用: 小鼠, 人, 大鼠 FACS, WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 21-290) antibodyEMR1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (C-Term) antibodyEMR1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 1-201) antibodyEMR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (C-Term) antibodyEMR1 适用: 小鼠, 人, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibody (PE)EMR1 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal BM8-1 PE
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 21-290) antibodyEMR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 1-821) antibodyEMR1 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibodyEMR1 适用: 小鼠 FACS, WB, IHC (p) 宿主: 大鼠 Monoclonal BM8 unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 626-886) antibodyEMR1 适用: 小鼠, 人, 大鼠, 犬 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Mouse macrophages control Negative Mouse fibroblasts or granulocytes control
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 1.0 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Inactivation of the F4/80 glycoprotein in the mouse germ line." in: Molecular and cellular biology, Vol. 22, Issue 22, pp. 8035-43, (2002) (PubMed).
: "Macrophage trafficking in the uterus and cervix precedes parturition in the mouse." in: Biology of reproduction, Vol. 61, Issue 4, pp. 879-83, (1999) (PubMed).
: "Markers of mouse macrophage development detected by monoclonal antibodies." in: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, (1994) (PubMed).
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: "Inactivation of the F4/80 glycoprotein in the mouse germ line." in: Molecular and cellular biology, Vol. 22, Issue 22, pp. 8035-43, (2002) (PubMed).
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- 抗原
- F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
- Abstract
- EMR1 产品
- 别名
- EMR1 antibody, TM7LN3 antibody, DD7A5-7 antibody, EGF-TM7 antibody, F4/80 antibody, Gpf480 antibody, Ly71 antibody, adhesion G protein-coupled receptor E1 antibody, ADGRE1 antibody, LOC100424335 antibody, Adgre1 antibody
- 背景
- The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 Molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. The monoclonal antibody BM8 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Immunogen BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
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