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小鼠 anti-人 IgG4 (pFc' Region) Antibody (TRITC)

WB, ELISA Monoclonal NI 315 (HP 6206) IgG1 kappa TRITC

产品编号 ABIN457904

发货至: 中国

Quick Overview for 小鼠 anti-人 IgG4 (pFc' Region) Antibody (TRITC) (ABIN457904)

抗原

IgG4

克隆类型

单克隆

标记

19
4
3
3
2
2
1
1
1
1
1
1
1
1

TRITC

应用范围

29
21
18
10
9
9
8
7
3
3
2
2
1
1
1
1

Western Blotting (WB), ELISA

克隆位点

NI 315 (HP 6206)

亚型

IgG1 kappa

抗原表位
8

6

5

1
pFc' Region
适用
41

2

1

1
人
宿主
29

11

1
小鼠

特异性

The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.

产品特性

Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG4, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 2.7.

纯化方法

Purified

免疫原

Highly purified monoclonal IgG4 isolated from human serum.
Rabbit anti-Human IgG4 (AA 222-327) antibody
WB, IHC, IP, IF/ICC Polyclonal unconjugated

Mouse anti-Human IgG4 (pFc' Region) antibody
ELISA Monoclonal HP6023 IgG3 unconjugated

Mouse anti-Human IgG4 (Fc Region) antibody
ELISA Monoclonal HP6025 IgG1 unconjugated

Mouse anti-Human IgG4 (Fc Region) antibody (PE)
ELISA Monoclonal HP6025 IgG1 PE

Mouse anti-Human IgG4 antibody
Animal-Free ELISA Monoclonal AB131-2-C08 IgG2a Recombinant Antibody unconjugated

Mouse anti-Chimpanzee IgG4 antibody
WB, IHC (fro), IHC (p) Monoclonal HP6025 IgG1 unconjugated

Mouse anti-Human IgG4 (pFc' Region) antibody (HRP)
WB, IHC, ELISA Monoclonal HP6023 IgG3 HRP

Mouse anti-Human IgG4 (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6025 IgG1 Biotin

Mouse anti-Human IgG4 (pFc' Region) antibody (Alkaline Phosphatase (AP))
WB, IHC, ELISA Monoclonal HP6023 IgG3 Alkaline Phosphatase (AP)

Mouse anti-Human IgG4 (Fc Region) antibody (HRP)
WB, IHC, ELISA Monoclonal HP6025 IgG1 HRP

应用备注

To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

浓度

0.4 mg/mL

缓冲液

Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).

储存液

Without preservative

注意事项

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

储存条件

4 °C/-20 °C

储存方法

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
抗原

IgG4

别名

Immunoglobulin G4 (IgG4)

物质类

Antibody

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