小鼠 anti-人 IgG4 (Fc Region) Antibody (HRP)
-
- 抗原 See all IgG4 products
- IgG4
-
抗原表位
- Fc Region
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- HRP
-
应用范围
- Western Blotting (WB), ELISA
- 特异性
- The reactivity of the antiserum is restricted to the subclass specific determinant on the pFc portion of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG4, subclass specific
- 产品特性
- Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
- 纯化方法
- Purified
- 免疫原
- Highly purified monoclonal IgG4 isolated from pooled human serum
- 克隆位点
- NI 315 (HP 6206)
- 亚型
- IgG1 kappa
-
Rabbit anti-Human IgG4 (AA 222-327) antibody
WB, IHC, IP, IF/ICC Polyclonal unconjugated
Mouse anti-Human IgG4 (Fc Region) antibodyELISA Monoclonal HP6025 IgG1 unconjugated
Mouse anti-Human IgG4 (pFc' Region) antibodyELISA Monoclonal HP6023 IgG3 unconjugated
Mouse anti-Human IgG4 (Fc Region) antibody (PE)ELISA Monoclonal HP6025 IgG1 PE
Mouse anti-Human IgG4 (pFc' Region) antibody (HRP)WB, ELISA, IHC Monoclonal HP6023 IgG3 HRP
Mouse anti-Human IgG4 (Fc Region) antibody (Biotin)ELISA Monoclonal HP6025 IgG1 Biotin
Mouse anti-Human IgG4 (pFc' Region) antibody (Alkaline Phosphatase (AP))WB, ELISA, IHC Monoclonal HP6023 IgG3 Alkaline Phosphatase (AP)
Mouse anti-Human IgG4 (pFc' Region) antibody (Biotin)ELISA Monoclonal HP6023 IgG3 Biotin
Mouse anti-Human IgG4 (Fc Region) antibody (HRP)WB, ELISA, IHC Monoclonal HP6025 IgG1 HRP
-
- 应用备注
-
To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. - 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
- 浓度
- 0.4 mg/mL
- 缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
- 储存液
- Without preservative
- 注意事项
-
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. - 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
-
- 抗原
- IgG4
- 别名
- Immunoglobulin G4 (IgG4) (IgG4 产品)
- 物质类
- Antibody
-
