EGF Protein (AA 971-1023)
Quick Overview for EGF Protein (AA 971-1023) (ABIN987814)
抗原
See all EGF 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 971-1023
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产品特性
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ED50 <0.6 ng/mL, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of >1.7 x 10^6 units/mg.
AA 971-1023, expressed with an N-terminal Met -
内毒素水平
- < 0.1 ng/μg (1 EU/μg) determined by LAL test
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Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)EGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in ddH2O at 100 μg/mL.
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缓冲液
- Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80 °C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4 °C or up to 6 months at -20 °C.
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有效期
- 12 months
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- EGF (Epidermal Growth Factor (EGF))
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别名
- Epidermal Growth Factor
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背景
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Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-a and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds. Recombinant human Epidermal Growth Factor (rhEGF) produced in E. coli is a non-glycosylated polypeptide chain of 54 amino acids. A fully biologically active molecule, rhEGF has a molecular mass of 6.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Urogastrone, URG -
分子量
- 6.2kDa, observed by reducing SDS-PAGE.
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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