KIT Ligand Protein (KITLG) (full length)
Quick Overview for KIT Ligand Protein (KITLG) (full length) (ABIN935785)
抗原
See all KIT Ligand (KITLG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- full length
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产品特性
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Purified recombinant Human SCF protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is -
纯化方法
- Proprietary chromatographic technique
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KIT Ligand (KITLG) (Active) protein
KITLG 宿主: 人 宿主: Yeast (Pichia pastoris) > 95 % by SDS-PAGE Active
KIT Ligand (KITLG) (AA 26-214) protein (Fc Tag)KITLG 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) protein (His tag)KITLG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-190) protein (His tag)KITLG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His-IF2DI Tag)KITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) protein (His tag)KITLG 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) (Active) proteinKITLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
KIT Ligand (KITLG) (AA 1-245) protein (GST tag)KITLG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Each Investigator should determine their own optimal working dilution for specific applications.
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说明
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Biological activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is
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实验流程
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Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in sterile water not less than 100 μg/mL.
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缓冲液
- Lyophilized from a concentrated (1 mg/mL) solution in water containing 11 mM CH3COOH.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- KIT Ligand (KITLG)
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别名
- SCF
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背景
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Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as steel factor or c-kit ligand. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
Alternative Names: SCF protein, MGF protein, Mast cell growth factor protein, Kitl protein, c-Kit Ligand protein, Steel Factor protein, KL-1 protein, MGF protein, C-kit ligand protein, SF protein, Mast Cell Growth Factor protein, Kit ligand Precursor protein, DKFZp686F2250, Stem Cell Factor protein -
分子量
- 18.409 Da
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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