Purified recombinant Human IFN gamma protein Expression System: E.coli Bioactivity: The ED50 as determined by a cytopathic affect inhibition assay with Mouse L929 cells challenged with EMC virus was 1 x 10^7 units/mg.
IFNG
宿主: Hamster
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % by SDS-PAGE gel analyses
WB, ELISA
应用备注
Each Investigator should determine their own optimal working dilution for specific applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 10 mM Na0 O4 pH7.2 to a concentration of 0.1-1.0 mg/mL. When dissolved, dilute further into aqueous buffered solutions.
缓冲液
Lyophilized from 10 mM Na3PO4, pH7.2.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
RT/-20 °C
POGO, BRAWERMAN, CHARGAFF: "New ribonucleic acid species associated wihin the formation of the photosynthetic apparatus in Euglena gracilis." in: Biochemistry, Vol. 1, pp. 128-31 (PubMed).
IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells. Alternative Names: IFN g protein, Immune Interferon protein, T cell interferon protein, IFN-g protein, MAF protein, type II interferon protein, Interferon gamma protein