custom-made
PCNA
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
PCNA
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
PCNA protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Supplied as a liquid in 20 mM Tris, pH 7.5, containing 0 mM EDTA, and 20 % glycerol.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
RT/-20 °C
储存方法
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
PCNA (Proliferating Cell Nuclear protein) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques. Alternative Names: PCNA protein, , Proliferating cell nuclear protein protein, MGC8367 protein, Proliferating cell nuclear protein protein