Interferon gamma Protein (IFNG) (AA 23-156)
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Quick Overview for Interferon gamma Protein (IFNG) (AA 23-156) (ABIN8056980)
抗原
See all Interferon gamma (IFNG) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 23-156
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原理
- Recombinant Rat IFN-γ Protein(Halo Tag)
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序列
- Gln23-Cys156
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Interferon gamma (IFNG) (AA 24-161) (Active) protein (Biotin,His-Avi Tag)
IFNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interferon gamma (IFNG) (AA 24-161) (Active) protein (His-Avi Tag)IFNG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interferon gamma (IFNG) (Active) proteinIFNG 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) (AA 1-166) protein (GST tag)IFNG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interferon gamma (IFNG) (AA 23-155) protein (His tag)IFNG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Interferon gamma (IFNG) (AA 24-161) (Active) proteinIFNG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, AcA Active
Interferon gamma (IFNG) (AA 24-161) (Active) protein (His tag)IFNG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, AcA Active
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG 宿主: Hamster 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Interferon gamma (IFNG)
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别名
- IFN-gamma
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背景
- Interferon gamma,IFNG,Immune interferon,Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL.Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
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分子量
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Calculated MW: 47.6 kDa
Observed MW: 50-55 kDa
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基因ID
- 25712
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UniProt
- P01581
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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