LGMN 蛋白
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北京 101111
Quick Overview for LGMN 蛋白 (ABIN7964651)
抗原
See all LGMN 蛋白蛋白类型
宿主
资源
纯度
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原理
- Legumain, Asparaginyl Endopeptidase (AEP), human, recombinant, Leishmania
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产品特性
- Legumain is a cysteine protease with a strict specificity for cleaving after asparagine and, to a lesser extent, aspartic acid residues. Therefore, it is synonymously named the asparaginyl endopeptidase or AEP. Its very strict substrate specificity makes it an ideal enzyme for example for digestion proteomics. Human legumain is synthesized as an inactive proenzyme (56 kDa) composed of the caspase-like catalytic AEP domain (36 kDa) and a C-terminal death-domain-like prodomain. Activation to the active AEP proceeds via the pH -dependent autocatalytic release of the C-terminal prodomain at acidic pH . While prolegumain is stable at neutral pH , AEP is stable at acidic pH (≤ 6.0) but will irreversibly unfold at near neutral pH conditions. The pH -optimum of its endopeptidase activity is at pH 5.5 with a strong preference for cleaving after asparagine residues. Hydrolysis of aspartyl peptide bonds is preferentially catalyzed at more acidic pH ( pH 4.0). In addition to its well established protease activity, legumain harbors a pH -dependent ligase activity at near neutral pH .
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Legumain (LGMN) (AA 1-433) protein (GST tag)
LGMN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Legumain (LGMN) (Active) protein (His tag)LGMN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 75 % as determined by SDS-PAGE Active
Legumain (LGMN) protein (His tag)LGMN 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Legumain (LGMN) (AA 18-433) protein (His-IF2DI Tag)LGMN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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说明
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Optional[Concentration Comments]: 500 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- supplied in 20 mM citric acid pH 4.0100 mM NaCl2 mM DTT
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Please centrifuge briefly before opening (volume ≤2 ml).,avoid freeze/thaw cycles
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储存条件
- -20 °C
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储存方法
- store at -20 °C avoid freeze/thaw cycles
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有效期
- 12 months
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- LGMN (Legumain (LGMN))
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别名
- Legumain
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分子量
- 36 kDa
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UniProt
- Q99538
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途径
- Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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