LGMN Protein (His tag)
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- 抗原 See all LGMN 蛋白
- LGMN (Legumain (LGMN))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This LGMN protein is labelled with His tag.
- 原理
- Recombinant Mouse Legumain/LGMN Protein (His Tag)(Active)
- 序列
- Val 18-Tyr 435
- 产品特性
- A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 75 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC).The specific activity is > 350 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)
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- Discover our top product LGMN 蛋白
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Legumain (LGMN) (AA 18-435) protein (His tag)
LGMN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Legumain (LGMN) (AA 18-433) protein (His tag)LGMN 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Legumain (LGMN) (AA 18-325) protein (His-IF2DI Tag)LGMN 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Legumain (LGMN) (AA 1-433) protein (GST tag)LGMN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Legumain (LGMN) (AA 18-323) protein (His tag)Crystallography grade LGMN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Legumain (LGMN) (AA 18-325) protein (His tag)Crystallography grade LGMN 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 25 mM Tris, 0.15M NaCl, 20 % Glycerol, pH 7.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- LGMN (Legumain (LGMN))
- 别名
- Legumain/LGMN (LGMN 产品)
- 别名
- AEP Protein, LGMN1 Protein, PRSC1 Protein, AI746452 Protein, AU022324 Protein, Prsc1 Protein, MGC64351 Protein, cb173 Protein, cb460 Protein, sb:cb173 Protein, zgc:76953 Protein, wu:fa98g05 Protein, wu:fj92g08 Protein, lgmn Protein, MGC88980 Protein, DELTA VACUOLAR PROCESSING ENZYME Protein, DELTAVPE Protein, delta vacuolar processing enzyme Protein, legumain Protein, legumain L homeolog Protein, delta vacuolar processing enzyme Protein, Legumain Protein, LGMN Protein, Lgmn Protein, lgmn.L Protein, lgmn Protein, DELTA-VPE Protein, MBOO_RS07785 Protein, Odosp_3582 Protein, LOC569153 Protein
- 背景
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Background: The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with a strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. In addition, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis.
Synonym: Legumain,Lgmn,Asparaginyl endopeptidase,Protease cysteine 1,Prsc1,AEP
- 分子量
- 49.8 kDa
- NCBI登录号
- NP_035305
- 途径
- Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
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