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Quick Overview for LGMN Protein (His tag) (ABIN7320693)
抗原
See all LGMN 蛋白蛋白类型
宿主
资源
纯度
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标记
- This LGMN protein is labelled with His tag.
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原理
- Recombinant Mouse Legumain/LGMN Protein (His Tag)
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序列
- Val 18-Tyr 435
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产品特性
- Recombinant Mouse Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Val18-Tyr435 is expressed with a 6His tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Legumain (LGMN) (AA 18-433) protein (His-IF2DI Tag)
LGMN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Legumain (LGMN) (Active) protein (His tag)LGMN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 75 % as determined by reducing SDS-PAGE. Active
Legumain (LGMN) (AA 1-433) protein (GST tag)LGMN 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 20 % Glycerol, pH 8.0.
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- LGMN (Legumain (LGMN))
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别名
- Legumain/LGMN
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背景
- AEP,Asparaginyl endopeptidase,Legumain,Lgmn,Protease cysteine 1,Prsc1,Mouse Legumain,also known as LGMN, is a cysteine protease belonging to peptidase family C13 and is expressed in kidney and placenta abundantly. LGMN has a strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. The mammalian legumain is involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. It plays a role in the regulation of cell proliferation via its role in EGFR degradation. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis. Mammalian legumain is inhibited by iodoacetamide and maleimides. Legumain activation appears to be autocatalytic and can be triggered by acidic pH .
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分子量
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Calculated MW: 48.7 kDa
Observed MW: 60 kDa
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NCBI登录号
- NP_035305
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途径
- Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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