PKC zeta 蛋白
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Quick Overview for PKC zeta 蛋白 (ABIN7939473)
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See all PKC zeta (PRKCZ) 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant PRKCZ protein
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 152,621.24 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (His tag)
custom-made PRKCZ 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (His tag)custom-made PRKCZ 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase C, zeta (PRKCZ) (AA 1-592) protein (GST tag)PRKCZ 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein Kinase C, zeta (PRKCZ) (AA 454-592) protein (His-IF2DI Tag)PRKCZ 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant PRKCZ protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
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别名
- PRKCZ
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背景
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Protein Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCZ (Protein Kinase C Zeta), a calcium- and diacylglycerol-independent serine/threonine-protein kinase. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.PRKCZ functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation.
Short Description: Recombinant PRKCZ protein was expressed in baculovirus expression system as the full length protein (accession number NP_002735.3) with a N-terminal FLAG tag. The molecular weight of the protein is 68.9 kDa.
Aliases: Protein Kinase C Zeta, PKC Zeta
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途径
- NF-kappaB Signaling, RTK signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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