PAK4 蛋白
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北京 101111
Quick Overview for PAK4 蛋白 (ABIN7939404)
抗原
See all PAK4 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant PAK4 protein
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P21-Activated Kinase 4 (PAK4) (AA 1-259) protein (His tag)
PAK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
P21-Activated Kinase 4 (PAK4) (AA 1-591) protein (His tag)PAK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
P21-Activated Kinase 4 (PAK4) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PAK4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant PAK4 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- PAK4 (P21-Activated Kinase 4 (PAK4))
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别名
- PAK4
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背景
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Protein Background: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. PAK4 phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. PAK4 phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. PAK4 phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. PAK4 phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers.
Short Description: Recombinant PAK4 protein was expressed in baculovirus expression system as the full length protein (accession number NP_001014831.1) with a N-terminal FLAG tag. The molecular weight of the protein is 65.3 kDa.
Aliases: P21 (RAC1) Activated Kinase 4
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途径
- RTK signaling
抗原
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