KIT Protein (AA 546-976)
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Quick Overview for KIT Protein (AA 546-976) (ABIN7939264)
抗原
See all KIT 蛋白蛋白类型
宿主
资源
应用范围
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产品特性
- AA 546-976
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原理
- Recombinant KIT / CD117 (546-976) protein
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Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-520) protein (His tag)
KIT 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-516) (Active) protein (Fc Tag)KIT 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 1-976) protein (GST tag)KIT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mast/stem Cell Growth Factor Receptor (KIT) protein (His tag)KIT 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Mast/stem Cell Growth Factor Receptor (KIT) (Active) protein (His tag)KIT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Mast/stem Cell Growth Factor Receptor (KIT) protein (Fc Tag)KIT 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Mast/stem Cell Growth Factor Receptor (KIT) (Active) protein (Fc Tag)KIT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-524) protein (His tag)KIT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-516) (Active) protein (His tag)KIT 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant KIT (546-976) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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别名
- KIT & CD117
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背景
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Protein Background: KIT (KIT Proto-Oncogene, Receptor Tyrosine Kinase) is a tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. KIT phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL and activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KIT promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Diseases associated with KIT include Gastrointestinal Stromal Tumor and Piebald Trait.
Short Description: Recombinant KIT (546-976) protein that includes amino acids 546-976 of human KIT protein (accession number NP_000213.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 50 kDa.
Aliases: KIT Proto-Oncogene, Receptor Tyrosine Kinase, CD117
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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