p21 蛋白
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北京 101111
Quick Overview for p21 蛋白 (ABIN7938737)
抗原
See all p21 (CDKN1A) 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant CDKN1A protein
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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-164) protein (GST tag)
CDKN1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-164) protein (His-IF2DI Tag)CDKN1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CDKN1A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Recombinant CDKN1A protein is in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5 % glycerol.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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别名
- CDKN1A
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背景
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Protein Background: CDKN1A (Cyclin-dependent kinase inhibitor 1) may be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest.
Short Description: Recombinant CDKN1A protein that includes amino acids 1-164 of human CDKN1A protein (accession number NP_000380.1) was expressed was expressed in E. coli cells with an N-terminal 6x His tag. The molecular weight of the protein is 20.05 kDa.
Aliases: Cyclin-dependent kinase inhibitor 1, also known as P21, CIP1, CDK1A, P21CIP1
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途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
抗原
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