HMGB1 Protein (Terminally oxidized)
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北京 101111
Quick Overview for HMGB1 Protein (Terminally oxidized) (ABIN7866190)
抗原
See all HMGB1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
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产品特性
- Terminally oxidized
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原理
- Terminally oxidized HMGB1, LPS-Free
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序列
- Terminally oxidized-HMGB1 consists of 215 amino acid: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
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产品特性
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HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells (Dumitriu et al. 2005). HMGB1 is essential for life: Hmgb1 knockout mice die shortly after birth.
The two different activities of HMGB1 as a chemoattractanct of motile cells and as inducer of cytokines have been attributed to different biochemical forms. Fully reduced HMGB1 (the form with chemoattractanct activity) is completely reduced; disulfide HMGB1 contains a disulfide bond between C23 and C45 (complete notation: HMGB1C23-C45C106h – Antoine J. et al 2014).
Terminally oxidized-HMGB1 has all the cysteines oxidized to sulfonates and has no activity, either as a chemoattractant or in cytokine stimulation (Kazama et al,2008 ;Yang et al, 2012). -
纯化方法
- Purified
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内毒素水平
- LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL
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Biological Activity Comment
- Terminally oxidized-HMGB1 has all the cysteines oxidized to sulfonates and has no activity, either as a chemoattractant or in cytokine stimulation
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) (Active) protein (Biotin,His tag)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
High Mobility Group Box 1 (HMGB1) (Fully reduced) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (C106S), (C23S), (C45S), (Non-oxidizable) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 宿主: 人 宿主: Baculovirus Recombinant > 90 % pure SDS
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl.
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缓冲液
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The protein is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request. -
储存条件
- 4 °C/-20 °C
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储存方法
- 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- High-mobility group protein B1 (HMGB1)
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背景
- HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.
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分子量
- 24.8 kDa
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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