HMGB1 Protein (AA 2-89)
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- 抗原 See all HMGB1 蛋白
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-89
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Recombinant Human High Mobility Group Protein B1/HMGB1
- 序列
- GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGETKKKF
- 产品特性
- Recombinant Human High Mobility Group Protein B1/HMGB1/BOXA is produced by our E. coli expression system. The target protein is expressed with sequence (G2-F89) of Human HMBG1.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product HMGB1 蛋白
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag,Biotin)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (Non-oxidizable) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: Hi-5 Cells Recombinant > 90 % by SDS - PAGE SDS
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 50 mMHepes,500 mMNaCl,0.5 mMDTT, pH 7.9 .
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- 别名
- High Mobility Group Protein B1/HMGB1 (HMGB1 产品)
- 别名
- HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein
- 背景
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HMGB1 belongs to the HMGB family and contains 2 HMG box DNA-binding domains. HMGB1 is a DNA binding proteins that associates with chromatin and has the ability to bend DNA. This nuclear protein organizes the DNA and regulates transcription. After binding HMGB1 bends the DNA, which conducts to the binding of other proteins. HMGB1 suports transcription of many genes in interactions with many transcription factors or cooperation nucleosoms looses packed DNA and increases the chromatin remodeling, contact with core histones changes the structure of nucleosoms. it involved in V(D)J recombination by acting as a cofactor of the RAG complex. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, stays in the nucleus, but hyperacetylation on lysine residues occurs to translocation into the cytosol.
Synonyms: High-mobility group protein B1, high-mobility group protein 1, HMG-1, amphoterin and HMGB1. - 分子量
- 10.5 kDa
- UniProt
- P09429
- 途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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