电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Nanog Protein (AA 1-305) (His tag)

custom-made NANOG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
产品编号 ABIN7564307
发货至: 中国
  • 抗原 See all Nanog (NANOG) 蛋白
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    蛋白类型
    Recombinant
    产品特性
    AA 1-305
    宿主
    • 11
    • 1
    • 1
    • 1
    小鼠
    资源
    • 9
    • 2
    • 2
    • 1
    HEK-293 Cells
    标记
    This Nanog protein is labelled with His tag.
    原理
    Custom-made recombinant Nanog Protein expressed in mammalian cells.
    序列
    MSVGLPGPHS LPSSEEASNS GNASSMPAVF HPENYSCLQG SATEMLCTEA ASPRPSSEDL PLQGSPDSST SPKQKLSSPE ADKGPEEEEN KVLARKQKMR TVFSQAQLCA LKDRFQKQKY LSLQQMQELS SILNLSYKQV KTWFQNQRMK CKRWQKNQWL KTSNGLIQKG SAPVEYPSIH CSYPQGYLVN ASGSLSMWGS QTWTNPTWSS QTWTNPTWNN QTWTNPTWSS QAWTAQSWNG QPWNAAPLHN FGEDFLQPYV QLQQNFSASD LEVNLEATRE SHAHFSTPQA LELFLNYSVT PPGEI Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    特异性
    If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
    产品特性
    Key Benefits:
    • Made to order protein - from design to production - by highly experienced protein experts.
    • Protein expressed in mammalian cells and purified in one-step affinity chromatography
    • The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    If you are not interested in a full length protein, please contact us for individual protein fragments.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
    纯度
    > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
    质量等级
    custom-made
    Top Product
    Discover our top product NANOG 蛋白
  • 应用备注
    We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    12 months
  • 抗原
    Nanog (NANOG) (Nanog Homeobox (NANOG))
    别名
    Nanog (NANOG 产品)
    背景
    Homeobox protein NANOG (ES cell-associated protein 4) (Early embryo specific expression NK-type homeobox protein) (Homeobox transcription factor Nanog),FUNCTION: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal (PubMed:25825768). Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (By similarity). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. {ECO:0000250|UniProtKB:Q9H9S0, ECO:0000269|PubMed:12787504, ECO:0000269|PubMed:12787505, ECO:0000269|PubMed:14728807, ECO:0000269|PubMed:15502159, ECO:0000269|PubMed:16518401, ECO:0000269|PubMed:16791199, ECO:0000269|PubMed:16801560, ECO:0000269|PubMed:21915945, ECO:0000269|PubMed:22988117, ECO:0000269|PubMed:25825768}.
    分子量
    34.2 kDa
    UniProt
    Q80Z64
    途径
    Stem Cell Maintenance
You are here:
客服