ASAP2
(ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 2 (ASAP2))
蛋白类型
Recombinant
产品特性
AA 1-958
宿主
小鼠
资源
HEK-293 Cells
标记
This ASAP2 protein is labelled with His tag.
原理
Made-to-order recombinant Asap2 Protein expressed in mammalian cells.
序列
MPDQISVSEF VAETHEDYKA PTASSFTTRT AQCRNTVAAI EEALDVDRMV LYKMKKSVKA INISGLAHVE NEEQYTQALE KFGGNCVCRD DPDLGSAFLK FSVFTKELTA LFKNLIQNMN NIISFPLDSL LKGDLKGVKG DLKKPFDKAW KDYETKITKI EKEKKEHAKL HGMIRTEISG AEIAEEMEKE RRFFQLQMCE YLLKVNEIKV KKGVDLLQNL IKYFHAQCNF FQDGLKAVES LKPSIETLST DLHTIKQAQD EERRQLIQLR DILKSALQVE QKESRRDSQL RQSTAYSLHQ PQGNKEHGTE RNGNLYKKSD GIRKVWQKRK CSVKNGFLTI SHGTANRPPA KLNLLTCQVK TNPEEKKCFD LISHDRTYHF QAEDEQECQI WMSVLQNSKE EALNNAFKGD DNTGENNIVQ ELTKEIISEV QRMTGNDVCC DCGAPDPTWL STNLGILTCI ECSGIHRELG VHYSRMQSLT LDVLGTSELL LAKNIGNAGF NEIMECCLPS EDPVKPNPGS DMIARKDYIT AKYMERRYAR KKHADTAAKL HSLCEAVKTR DIFGLLQAYA DGVDLTEKIP LANGHEPDET ALHLAVRSVD RTSLHIVDFL VQNSGNLDKQ TGKGSTALHY CCLTDNAECL KLLLRGKASI EIANESGETP LDIAKRLKHE HCEELLTQAL SGRFNSHVHV EYEWRLLHED LDESDDDVDE KLQPSPNRRE DRPVSFYQLG SSQFQSNAVS LARDTANLTK DKQRGFGPSI LQNETYGAIL SGSPPSSQSI PPSTTSAPPL PPRNVGKDPL TTTPPPPVAK TSGTLEAMNQ PSKSSQPGTS QSKPPPLPPQ PPSRLPQKKP ASGTDKPTPL TNKGQPRGPE ASGPLSNAMA LQPPAPMPRK SQATKSKPKR VKALYNCVAD NPDELTFSEG DVIIVDGEED QEWWIGHIDG EPSRKGAFPV SFVHFIAD Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
ASAP2
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
ASAP2
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
ASAP2
(ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 2 (ASAP2))
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (Development and differentiation-enhancing factor 2) (Paxillin-associated protein with ARF GAP activity 3) (PAG3) (Pyk2 C-terminus-associated protein) (PAP),FUNCTION: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration (By similarity). {ECO:0000250, ECO:0000269|PubMed:10022920, ECO:0000269|PubMed:11304556}.