DAXX Protein (AA 1-739) (His tag)
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北京 101111
Quick Overview for DAXX Protein (AA 1-739) (His tag) (ABIN7562341)
抗原
See all DAXX 蛋白蛋白类型
宿主
资源
纯度
质量等级
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产品特性
- AA 1-739
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标记
- This DAXX protein is labelled with His tag.
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原理
- Predefined custom protein recombinant Daxx Protein expressed in mammalian cells.
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序列
- MATDDSIIVL DDDDEDEAAA QPGPSNLPPN PASTGPGPGL SQQATGLSEP RVDGGSSNSG SRKCYKLDNE KLFEEFLELC KTETSDHPEV VPFLHKLQQR AQSVFLASAE FCNILSRVLA RSRKRPAKIY VYINELCTVL KAHSIKKKLN LAPAASTTSE ASGPNPPTEP PSDLTNTENT ASEASRTRGS RRQIQRLEQL LALYVAEIRR LQEKELDLSE LDDPDSSYLQ EARLKRKLIR LFGRLCELKD CSSLTGRVIE QRIPYRGTRY PEVNRRIERL INKPGLDTFP DYGDVLRAVE KAATRHSLGL PRQQLQLLAQ DAFRDVGVRL QERRHLDLIY NFGCHLTDDY RPGVDPALSD PTLARRLREN RTLAMNRLDE VISKYAMMQD KTEEGERQKR RARLLGTAPQ PSDPPQASSE SGEGPSGMAS QECPTTSKAE TDDDDDDDDD DDEDNEESEE EEEEEEEEKE ATEDEDEDLE QLQEDQGGDE EEEGGDNEGN ESPTSPSDFF HRRNSEPAEG LRTPEGQQKR GLTETPASPP GASLDPPSTD AESSGEQLLE PLLGDESPVS QLAELEMEAL PEERDISSPR KKSEDSLPTI LENGAAVVTS TSVNGRVSSH TWRDASPPSK RFRKEKKQLG SGLLGNSYIK EPMAQQDSGQ NTSVQPMPSP PLASVASVAD SSTRVDSPSH ELVTSSLCSP SPSLLLQTPQ AQSLRQCIYK TSVATQCDPE EIIVLSDSD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
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特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
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产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
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Death-Domain Associated Protein (DAXX) (AA 1-740) protein (His tag)
custom-made DAXX 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Death-Domain Associated Protein (DAXX) (AA 1-740) protein (GST tag)DAXX 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Death-Domain Associated Protein (DAXX) (AA 1-233), (partial) protein (GST tag)DAXX 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Death-Domain Associated Protein (DAXX) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DAXX 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Death-Domain Associated Protein (DAXX) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DAXX 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Death-Domain Associated Protein (DAXX) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)DAXX 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The buffer composition is at the discretion of the manufacturer.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- DAXX (Death-Domain Associated Protein (DAXX))
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别名
- Daxx
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背景
- Death domain-associated protein 6 (Daxx),FUNCTION: Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity, the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as a histone chaperone that facilitates deposition of histone H3.3. Acts as a targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs), the process is independent of ATRX and facilitated by ASF1A, PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Plays a role as a positive regulator of the heat shock transcription factor HSF1 activity during the stress protein response (By similarity). {ECO:0000250|UniProtKB:Q9UER7, ECO:0000269|PubMed:10684855, ECO:0000269|PubMed:20651253, ECO:0000269|PubMed:22500635}.
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分子量
- 81.5 kDa
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UniProt
- O35613
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AlphaFold
- O35613
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
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