LARP7 Protein (AA 1-570) (His tag)
custom-made
LARP7
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 抗原 See all LARP7 蛋白
- LARP7 (La Ribonucleoprotein Domain Family, Member 7 (LARP7))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-570
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This LARP7 protein is labelled with His tag.
- 原理
- Made-to-order recombinant Larp7 Protein expressed in mammalian cells.
- 序列
- METENQKTME ESTKRKEEKK KRSRVKQVLA DIAKQVDFWF GDANLHKDKF LREQIEKSRD GYVDISLLVS FNKMKKLTTD GKLIARALKS SSVVELDLEG TRIRRKKPLG ERPKDEEERT VYVELLPKNV THSWIERVFG KCGNVVYISI PHYKSTGDPK GFAFVEFETK EQAAKAIEFL NNPPEEAPRK PGIFPKTVKN KPIPSLRVAE EKKKKKKKKG RIKKEESVQA KESAVDSSSS GVCKATKRPR TASEGSEAET PEAPKQPAKK KKKRDKVEAS SLPEARAGKR ERCSAEDEDC LPPRPKAKKR AQKDGVGQAA SEVSKESRDL EFCSTEEEKE TDRKGDSLSK VKRKHKKKHK ERHKMGEEVI PLRVLSKTEW MDLKKEYLAL QKASMASLKK TISQIKLESE METDCKAPTA GSGQECSTQE KVSAQGPQFV TGVIVKIVSG EPLPGRKQVK DILATISEVV YIDLLEGDTE CHARFKTPED AQAVMNAQTE IRKKHSWNLE VLSGDHEQRY WQKILVDRQA KLNQPREKKR GTEKLITKAE KIRLAKTQQA SQHIRFSEYD Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
- Top Product
- Discover our top product LARP7 蛋白
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La Ribonucleoprotein Domain Family, Member 7 (LARP7) (AA 1-582) protein (His tag)
custom-made LARP7 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
La Ribonucleoprotein Domain Family, Member 7 (LARP7) (AA 1-31) protein (GST tag)LARP7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- LARP7 (La Ribonucleoprotein Domain Family, Member 7 (LARP7))
- 别名
- Larp7 (LARP7 产品)
- 背景
- La-related protein 7 (La ribonucleoprotein domain family member 7),FUNCTION: RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function (PubMed:32017896). Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II (By similarity). The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (By similarity). The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (By similarity). LARP7 specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA, on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex (By similarity). LARP7 also acts as a regulator of mRNA splicing fidelity by promoting U6 snRNA processing (PubMed:32017896). Specifically binds U6 snRNAs and associates with a subset of box C/D RNP complexes: promotes U6 snRNA 2'-O-methylation by facilitating U6 snRNA loading into box C/D RNP complexes (PubMed:32017896). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017896). Binds U6 snRNAs with a 5'-CAGGG-3' sequence motif (By similarity). U6 snRNA processing is required for spermatogenesis (PubMed:32017896). {ECO:0000250|UniProtKB:Q4G0J3, ECO:0000269|PubMed:32017896}.
- 分子量
- 64.8 kDa
- UniProt
- Q05CL8
- AlphaFold
- Q05CL8
- 途径
- Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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