ASK1 Protein (AA 1-1374) (His tag)
Quick Overview for ASK1 Protein (AA 1-1374) (His tag) (ABIN7554476)
抗原
See all ASK1 (MAP3K5) 蛋白蛋白类型
宿主
资源
纯度
质量等级
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产品特性
- AA 1-1374
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标记
- This ASK1 protein is labelled with His tag.
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原理
- Predefined custom protein recombinant MAP3K5 Protein expressed in mammalian cells.
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序列
- MSTEADEGIT FSVPPFAPSG FCTIPEGGIC RRGGAAAVGE GEEHQLPPPP PGSFWNVESA AAPGIGCPAA TSSSSATRGR GSSVGGGSRR TTVAYVINEA SQGQLVVAES EALQSLREAC ETVGATLETL HFGKLDFGET TVLDRFYNAD IAVVEMSDAF RQPSLFYHLG VRESFSMANN IILYCDTNSD SLQSLKEIIC QKNTMCTGNY TFVPYMITPH NKVYCCDSSF MKGLTELMQP NFELLLGPIC LPLVDRFIQL LKVAQASSSQ YFRESILNDI RKARNLYTGK ELAAELARIR QRVDNIEVLT ADIVINLLLS YRDIQDYDSI VKLVETLEKL PTFDLASHHH VKFHYAFALN RRNLPGDRAK ALDIMIPMVQ SEGQVASDMY CLVGRIYKDM FLDSNFTDTE SRDHGASWFK KAFESEPTLQ SGINYAVLLL AAGHQFESSF ELRKVGVKLS SLLGKKGNLE KLQSYWEVGF FLGASVLAND HMRVIQASEK LFKLKTPAWY LKSIVETILI YKHFVKLTTE QPVAKQELVD FWMDFLVEAT KTDVTVVRFP VLILEPTKIY QPSYLSINNE VEEKTISIWH VLPDDKKGIH EWNFSASSVR GVSISKFEER CCFLYVLHNS DDFQIYFCTE LHCKKFFEMV NTITEEKGRS TEEGDCESDL LEYDYEYDEN GDRVVLGKGT YGIVYAGRDL SNQVRIAIKE IPERDSRYSQ PLHEEIALHK HLKHKNIVQY LGSFSENGFI KIFMEQVPGG SLSALLRSKW GPLKDNEQTI GFYTKQILEG LKYLHDNQIV HRDIKGDNVL INTYSGVLKI SDFGTSKRLA GINPCTETFT GTLQYMAPEI IDKGPRGYGK AADIWSLGCT IIEMATGKPP FYELGEPQAA MFKVGMFKVH PEIPESMSAE AKAFILKCFE PDPDKRACAN DLLVDEFLKV SSKKKKTQPK LSALSAGSNE YLRSISLPVP VLVEDTSSSS EYGSVSPDTE LKVDPFSFKT RAKSCGERDV KGIRTLFLGI PDENFEDHSA PPSPEEKDSG FFMLRKDSER RATLHRILTE DQDKIVRNLM ESLAQGAEEP KLKWEHITTL IASLREFVRS TDRKIIATTL SKLKLELDFD SHGISQVQVV LFGFQDAVNK VLRNHNIKPH WMFALDSIIR KAVQTAITIL VPELRPHFSL ASESDTADQE DLDVEDDHEE QPSNQTVRRP QAVIEDAVAT SGVSTLSSTV SHDSQSAHRS LNVQLGRMKI ETNRLLEELV RKEKELQALL HRAIEEKDQE IKHLKLKSQP IEIPELPVFH LNSSGTNTED SELTDWLRVN GADEDTISRF LAEDYTLLDV LYYVTRDDLK CLRLRGGMLC TLWKAIIDFR NKQT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
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特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
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产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 4,244.43 ¥表达系统 Insect CellsConjugate GST tagOrigin HumanPrice 3,685.50 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 4,660.84 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 6,047.67 ¥表达系统 HEK-293 CellsConjugate Myc-DYKDDDDK TagOrigin HumanPrice 8,289.22 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1-1380) protein (His tag)
custom-made MAP3K5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1180-1374) protein (His tag)MAP3K5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) proteinMAP3K5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) protein (Myc-DYKDDDDK Tag)MAP3K5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The buffer composition is at the discretion of the manufacturer.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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别名
- MAP3K5
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背景
- Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) (Apoptosis signal-regulating kinase 1) (ASK-1) (MAPK/ERK kinase kinase 5) (MEK kinase 5) (MEKK 5),FUNCTION: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1). {ECO:0000269|PubMed:10411906, ECO:0000269|PubMed:10688666, ECO:0000269|PubMed:10849426, ECO:0000269|PubMed:11029458, ECO:0000269|PubMed:11154276, ECO:0000269|PubMed:11689443, ECO:0000269|PubMed:11920685, ECO:0000269|PubMed:14688258, ECO:0000269|PubMed:14749717, ECO:0000269|PubMed:15023544, ECO:0000269|PubMed:16129676, ECO:0000269|PubMed:17220297, ECO:0000269|PubMed:23102700, ECO:0000269|PubMed:26095851, ECO:0000269|PubMed:8940179, ECO:0000269|PubMed:8974401, ECO:0000269|PubMed:9564042, ECO:0000269|PubMed:9774977}.
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分子量
- 154.5 kDa
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UniProt
- Q99683
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AlphaFold
- Q99683
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途径
- MAPK Pathway, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
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