PCNA 蛋白
Quick Overview for PCNA 蛋白 (ABIN7536214)
抗原
See all PCNA 蛋白蛋白类型
宿主
资源
纯度
-
-
原理
- Recombinant Human Proliferating cell nuclear antigen/PCNA Protein
-
序列
- FEARLVQGSI LKKVLEALKD LINEACWDIS SSGVNLQSMD SSHVSLVQLT LRSEGFDTYR CDRNLAMGVN LTSMSKILKC AGNEDIITLR AEDNADTLAL VFEAPNQEKV SDYEMKLMDL DVEQLGIPEQ EYSCVVKMPS GEFARICRDL SHIGDAVVIS CAKDGVKFSA SGELGNGNIK LSQTSNVDKE EEAVTIEMNE PVQLTFALRY LNFFTKATPL SSTVTLSMSA DVPLVVEYKI ADMGHLKYYL APKIEDEEGS
-
特异性
- Phe2-Ser261
-
过滤
- 0.22 μm filtered
-
内毒素水平
- <0.1EU/μg
-
-
想要此Protein的其他选项吗？
!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 172,528.32 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) protein (His tag)
custom-made PCNA 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) protein (His tag)custom-made PCNA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) protein (GST tag)PCNA 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) proteinPCNA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
-
浓度
- 1.6 mg/mL
-
缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
-
-
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
-
别名
- Proliferating cell nuclear antigen/PCNA
-
背景
-
Description: Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
Name: ATLD2,Proliferating cell nuclear antigen,PCNA
-
基因ID
- 5111
-
UniProt
- P12004
-
途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
抗原
-
