IGF1 蛋白
Quick Overview for IGF1 蛋白 (ABIN7535993)
抗原
See all IGF1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Active Recombinant Human IGF-I Protein
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序列
- GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
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特异性
- Gly49-Ala118
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过滤
- 0.22 μm filtered
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内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 53.5-214.2 ng/mL.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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缓冲液
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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别名
- IGF-I
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背景
- IGF-1,IGF-I,IGF1A,IGFI,IGF?I,MGF,IGF1
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基因ID
- 3479
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UniProt
- P05019-1
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途径
- RTK signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
抗原
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