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CD80 Protein (CD80) (His tag)

This Recombinant CD80 protein is expressed in HEK-293 Cells.
产品编号 ABIN7533837
发货至: 中国

Quick Overview for CD80 Protein (CD80) (His tag) (ABIN7533837)

抗原

See all CD80 蛋白
CD80

蛋白类型

Recombinant

生物活性

Active

宿主

  • 28
  • 18
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资源

  • 27
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HEK-293 Cells

纯度

> 92 % by SDS-PAGE.
  • 标记

    This CD80 protein is labelled with His tag.

    原理

    Active Recombinant Human B7-1/CD80 Protein

    序列

    VIHVTKEVKE VATLSCGHNV SVEELAQTRI YWQKEKKMVL TMMSGDMNIW PEYKNRTIFD ITNNLSIVIL ALRPSDEGTY ECVVLKYEKD AFKREHLAEV TLSVKADFPT PSISDFEIPT SNIRRIICST SGGFPEPHLS WLENGEELNA INTTVSQDPE TELYAVSSKL DFNMTTNHSF MCLIKYGHLR VNQTFNWNTT KQEHFPDN

    特异性

    Val35-Asn242

    过滤

    0.22 μm filtered

    内毒素水平

    < 0.1 EU/μg of the protein by LAL method.

    Biological Activity Comment

    Measured by its binding ability in a functional ELISA. Immobilized Recombinant human CD80 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA-4 with a linear range of 10-50 ng/mL.
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

    浓度

    1 mg/mL

    缓冲液

    Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

    储存条件

    -20 °C,-80 °C

    储存方法

    Store the lyophilized protein at -20°C to -80 °C for long term.
    After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
  • 抗原

    CD80

    别名

    B7-1/CD80

    背景

    Description: The protein is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

    Name: B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7,CD80

    基因ID

    941

    UniProt

    P33681

    途径

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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