PLAT Protein (AA 33-559) (hFc Tag)
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Quick Overview for PLAT Protein (AA 33-559) (hFc Tag) (ABIN7504553)
抗原
See all PLAT 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 33-559
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标记
- This PLAT protein is labelled with hFc Tag.
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原理
- Mouse tPA Protein
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序列
- Ser33-Gln559
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产品特性
- Recombinant Mouse tPA Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Ser33-Gln559, which consists of two chains: chain A (Ser36-Arg308) and chain B (Ile309-Gln559).
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse tPA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 200 pmol/min/ug.
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Plasminogen Activator, Tissue (PLAT) protein (Fc Tag)
PLAT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
Plasminogen Activator, Tissue (PLAT) (AA 36-562) protein (His tag)PLAT 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Plasminogen Activator, Tissue (PLAT) (AA 311-562) protein (His tag)PLAT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % SDS
Plasminogen Activator, Tissue (PLAT) (AA 1-562) protein (GST tag)PLAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Plasminogen Activator, Tissue (PLAT) (AA 24-562) protein (His tag)PLAT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Plasminogen Activator, Tissue (PLAT) (AA 36-310) protein (His tag)PLAT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- PLAT (Plasminogen Activator, Tissue (PLAT))
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别名
- tPA
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背景
- Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
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分子量
- 85.00 kDa. It migrates to 35-40 kDa and 30-35 kDa based on Bis-Tris PAGE result.
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UniProt
- P11214
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途径
- Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
抗原
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