PLAT Protein (AA 24-562) (His tag)
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北京 101111
Quick Overview for PLAT Protein (AA 24-562) (His tag) (ABIN6387935)
抗原
See all PLAT 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 24-562
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标记
- This PLAT protein is labelled with His tag.
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序列
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QEIHARFRRG ARSYQVICRD EKTQMIYQQH QSWLRPVLRS NRVEYCWCNS GRAQCHSVPV KSCSEPRCFN GGTCQQALYF SDFVCQCPEG FAGKCCEIDT RATCYEDQGI SYRGTWSTAE SGAECTNWNS SALAQKPYSG RRPDAIRLGL GNHNYCRNPD RDSKPWCYVF KAGKYSSEFC STPACSEGNS DCYFGNGSAY RGTHSLTESG ASCLPWNSMI LIGKVYTAQN PSAQALGLGK HNYCRNPDGD AKPWCHVLKN RRLTWEYCDV PSCSTCGLRQ YSQPQFRIKG GLFADIASHP WQAAIFAKHR RSPGERFLCG GILISSCWIL SAAHCFQERF PPHHLTVILG RTYRVVPGEE EQKFEVEKYI VHKEFDDDTY DNDIALLQLK SDSSRCAQES SVVRTVCLPP ADLQLPDWTE CELSGYGKHE ALSPFYSERL KEAHVRLYPS SRCTSQHLLN RTVTDNMLCA GDTRSGGPQA NLHDACQGDS GGPLVCLNDG RMTLVGIISW GLGCGQKDVP GVYTKVTNYL DWIRDNMRP
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纯化方法
- > 90% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Plasminogen Activator, Tissue (PLAT) protein (Fc Tag)
PLAT 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
Plasminogen Activator, Tissue (PLAT) (AA 36-562) protein (His tag)PLAT 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Plasminogen Activator, Tissue (PLAT) (AA 311-562) protein (His tag)PLAT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % SDS
Plasminogen Activator, Tissue (PLAT) (AA 1-562) protein (GST tag)PLAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Plasminogen Activator, Tissue (PLAT) (AA 36-310) protein (His tag)PLAT 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- 50 mM MES buffer ( pH 5.5) containing 40 % glycerol, 5 mM CaCl2, 1 mM DTT, 0.5M NaCl
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PLAT (Plasminogen Activator, Tissue (PLAT))
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别名
- t-Plasminogen Activator/PLAT
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背景
- PLAT, as known as tissue-type plasminogen activator isoform 1, is a secreted serine protease synthesized by endothelial cells. This protein is secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Also, it plays a role clinical medicine to treat stroke in cell migration and tissue remodeling. It is implicated in normal neural function and is suggested as a regulatory molecule in neurodegenerative diseases. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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分子量
- 61.3 kDa (545aa)
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NCBI登录号
- NP_000921
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途径
- Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
抗原
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