Interferon gamma Protein (IFNG) (AA 24-166)
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Quick Overview for Interferon gamma Protein (IFNG) (AA 24-166) (ABIN7504424)
抗原
See all Interferon gamma (IFNG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 24-166
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原理
- Human IFN gamma/IFNG Protein
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序列
- Gln24-Gln166
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产品特性
- Recombinant Human IFN gamma/IFNG Protein is expressed from E.coli without tag.It contains Gln24-Gln166.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 0.01 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IFN gamma, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA (QC Test).,Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IFN gamma is greater than 90% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in 50 mM Tris ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Interferon gamma (IFNG)
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别名
- IFN gamma
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背景
- Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.
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分子量
- 16.77 kDa same as Bis-Tris PAGE result.
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UniProt
- P01579
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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