Pro BDNF Protein (Biotin)
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北京 101111
Quick Overview for Pro BDNF Protein (Biotin) (ABIN7491800)
抗原
蛋白类型
生物活性
宿主
资源
应用范围
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标记
- This Pro BDNF protein is labelled with Biotin.
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原理
- human proBDNF-Biotin
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序列
- MAPMKEANIR GQGGLAYPGV RTHGTLESVN GPKAGSRGLT SLADTFEHVI EELLDEDQKV RPNEENNKDA DLYTSRVMLS SQVPLEPPLL FLLEEYKNYL DAANMSMRVAGHSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR (the prodomain is shown in bold, underlined are the double point mutations preventing cleavage)
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特异性
- Toxin target: p75 neurotrophin receptor (p75NTR), Sortilin receptors
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产品特性
- LC-Biotin. The extent of labeling is 1-3 Molecules of biotin per one molecule of human proBDNF, linked via primary amines.
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Biological Activity Comment
- proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons expressing both p75 and sortilin.
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Pro-Brain-Derived Neurotrophic Factor (proBDNF) protein
proBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Pro-Brain-Derived Neurotrophic Factor (proBDNF) (Active) proteinproBDNF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant ≥98 % (HPLC) WB, in vivo, in vitro Active
Pro-Brain-Derived Neurotrophic Factor (proBDNF) (V66M) (Active) proteinproBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant ≥98 % (HPLC) WB, CC Active
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应用备注
- Antigen Preadsorption Control: 0.1 - 10 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))
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别名
- proBDNF
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背景
- A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
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分子量
- ~52 kDa (dimer)
抗原
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