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Quick Overview for Pro BDNF 蛋白 (ABIN7491767)
抗原
蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Recombinant mouse proBDNF protein
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序列
- MAPMKEVNVH GQGNLAYPGV RTHGTLESVN GPRAGSRGLT TTSLADTFEH VIEELLDEDQ KVRPNEENHK DADLYTSRVM LSSQVPLEPP LLFLLEEYKN YLDAANMSMR VRRHSDPARR GELSVCDSIS EWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG CRGIDKRHWN SQCRTTQSYV RALTMDSKKR IGWRFIRIDT SCVCTLTIKR GR (the prodomain is shown in bold)
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特异性
- Toxin target: p75 neurotrophin receptor (p75NTR), Sortilin receptors
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内毒素水平
- <0.1 EU per 1 μg of the protein by the LAL method.
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Biological Activity Comment
- BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2. BDNF supports the survival of many cell types3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons9 expressing both p75 and sortilin, and to be involved in LTD10.
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Pro-Brain-Derived Neurotrophic Factor (proBDNF) protein
proBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Pro-Brain-Derived Neurotrophic Factor (proBDNF) (Active) protein (Biotin)proBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant WB, CC, LCI Active
Pro-Brain-Derived Neurotrophic Factor (proBDNF) (V66M) (Active) proteinproBDNF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant ≥98 % (HPLC) WB, CC Active
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应用备注
- Antigen Preadsorption Control: 0.2 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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- Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))
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别名
- proBDNF
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背景
- A recombinant mouse proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
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分子量
- 52.4 kDa (dimer)
抗原
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