VEGF121 Protein (AA 27-147)
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北京 101111
Quick Overview for VEGF121 Protein (AA 27-147) (ABIN7454231)
抗原
See all VEGF121 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 27-147
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原理
- Human VEGF121 Protein
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序列
- Ala27-Arg147
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产品特性
- Recombinant Human VEGF121 Protein is expressed from HEK293 without tag.It contains Ala27-Arg147.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 19.1ng/ml determined by ELISA.,Immobilized Human VEGF121, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-VEGF121 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.,Human VEGF121 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL.,The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC.
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Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) (Active) protein (Biotin,His-Avi Tag)
VEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) (Active) protein (His-Avi Tag)VEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 27-147) (Active) proteinVEGF121 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Cell Growth Factor 121 (VEGF121) (AA 28-147) (Active) protein (His tag)VEGF121 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % CC, AcA Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- VEGF121 (Vascular Endothelial Cell Growth Factor 121 (VEGF121))
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别名
- VEGF121
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背景
- Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
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分子量
- 14.07 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.
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UniProt
- P15692
抗原
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