CD44 Protein (CD44) (AA 21-220) (His tag)
Quick Overview for CD44 Protein (CD44) (AA 21-220) (His tag) (ABIN7454096)
抗原
See all CD44 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 21-220
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标记
- This CD44 protein is labelled with His tag.
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原理
- Human CD44 Protein
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序列
- Gln21-Pro220 & Gln386-Ala427
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产品特性
- Recombinant Human CD44 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Pro220 & Gln386-Ala427.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human CD44, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
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CD44 (CD44) (AA 1-699) protein (GST tag)
CD44 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
CD44 (CD44) (AA 152-311) protein (His-IF2DI Tag)CD44 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
CD44 (CD44) (AA 1-361) protein (GST tag)CD44 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
CD44 (CD44) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CD44 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- CD44
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别名
- CD44
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背景
- CD44 is a hyaluronan binding cell surface signal transducing receptor that influences motility, cell survival and proliferation as well as the formation of tumor microenvironment. CD44 contains two variable regions encoded by variable exons. Alternative splicing, which is often deregulated in cancer, can produce various isoforms of CD44 with properties that may have different tissue specific effects and therefore even diverse effects on cancer progression
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分子量
- 27.91 kDa. Due to glycosylation, the protein migrates to 45-68 kDa based on Tris-Bis PAGE result.
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UniProt
- P16070-1
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途径
- Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
抗原
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