Transferrin Protein (TF) (AA 20-698) (His tag)
Quick Overview for Transferrin Protein (TF) (AA 20-698) (His tag) (ABIN7275694)
抗原
See all Transferrin (TF) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 20-698
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标记
- This Transferrin protein is labelled with His tag.
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原理
- Human Transferrin Protein
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序列
- Val20-Pro698
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产品特性
- Recombinant Human Transferrin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Pro698.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Transferrin (TF) (AA 1-698) protein (GST tag)
TF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin (TF) proteinTF 宿主: 小鼠 宿主: 小鼠 Native WB, ELISA, IHC
Transferrin (TF) (Active) protein (Fc Tag)TF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Transferrin (TF) (Active) protein (His tag)TF 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Transferrin (TF) protein (FITC)TF 宿主: 人 宿主: Human IF
Transferrin (TF) protein (FITC)TF 宿主: 人 宿主: Human Native DB, FM
Transferrin (TF) protein (Biotin)TF 宿主: 人 宿主: Human Native WB, ELISA, DB, IHC, IM
Transferrin (TF) (AA 20-697) protein (Fc Tag)TF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Transferrin (TF)
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别名
- Transferrin
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背景
- Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain, i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.
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分子量
- 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Tris-Bis PAGE result.
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途径
- Transition Metal Ion Homeostasis
抗原
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