Transferrin Protein (TF) (AA 1-698) (His tag)
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Quick Overview for Transferrin Protein (TF) (AA 1-698) (His tag) (ABIN7321071)
抗原
See all Transferrin (TF) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-698
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标记
- This Transferrin protein is labelled with His tag.
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原理
- Recombinant Rat Transferrin/TF Protein (His Tag)
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序列
- Met1-Ser698
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产品特性
- A DNA sequence encoding the rat TF (NP_001013128.1) (Met1-Ser698) was expressed with a polyhistidine tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K. P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.02-1 μg/mL.
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Transferrin (TF) (AA 1-698) protein (GST tag)
TF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transferrin (TF) proteinTF 宿主: 小鼠 宿主: 小鼠 Native WB, ELISA, IHC
Transferrin (TF) protein (FITC)TF 宿主: 人 宿主: Human IF
Transferrin (TF) (AA 1-697) (Active) protein (Fc Tag)TF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. CC Active
Transferrin (TF) protein (FITC)TF 宿主: 人 宿主: Human Native DB, FM
Transferrin (TF) (AA 20-697) protein (Fc Tag)TF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Transferrin (TF) protein (Biotin)TF 宿主: 人 宿主: Human Native WB, ELISA, DB, IHC
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.2
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Transferrin (TF)
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别名
- Transferrin/TF
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背景
- TF,Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte / pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins which bind Fe3+ ion in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.
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分子量
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Calculated MW: 76 kDa
Observed MW: 89 kDa
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基因ID
- 24825
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NCBI登录号
- NP_001013128
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UniProt
- P12346
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途径
- Transition Metal Ion Homeostasis
抗原
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