Notch1 Protein (AA 19-526) (His-Avi Tag,Biotin)
Quick Overview for Notch1 Protein (AA 19-526) (His-Avi Tag,Biotin) (ABIN7275364)
抗原
See all Notch1 (NOTCH1) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 19-526
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标记
- This Notch1 protein is labelled with His-Avi Tag,Biotin.
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原理
- Biotinylated Human Notch 1 Protein
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序列
- Ala19-Gln526
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产品特性
- Recombinant Biotinylated Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Notch 1 (NOTCH1) (AA 19-526) protein (His-Avi Tag)
NOTCH1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Notch 1 (NOTCH1) protein (His tag)NOTCH1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Notch 1 (NOTCH1) (AA 2309-2555) protein (His tag)NOTCH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Notch 1 (NOTCH1) (Active) protein (His tag)NOTCH1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 82 % as determined by SDS-PAGE Active
Notch 1 (NOTCH1) (AA 23-132) protein (GST tag)NOTCH1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Notch 1 (NOTCH1) (AA 19-526) (Active) protein (His tag)NOTCH1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Notch 1 (NOTCH1) (AA 19-526) (Active) protein (Fc Tag)NOTCH1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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别名
- Notch 1
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背景
- Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
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分子量
- 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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UniProt
- P46531
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途径
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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