MASP2 Protein (AA 288-686) (His tag)
Quick Overview for MASP2 Protein (AA 288-686) (His tag) (ABIN7275292)
抗原
See all MASP2 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 288-686
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标记
- This MASP2 protein is labelled with His tag.
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原理
- Human MASP2 Protein
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序列
- Gly288-Phe686
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产品特性
- Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)
MASP2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-686) protein (His tag)MASP2 宿主: Cynomolgus 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag,Biotin)MASP2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 1-686) protein (GST tag)MASP2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Mannan-Binding Lectin serine Peptidase 2 (MASP2) protein (Fc Tag)MASP2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
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别名
- MASP2
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背景
- The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.
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分子量
- 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.
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UniProt
- O00187-1
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途径
- Complement System
抗原
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