LIFR Protein (AA 45-833) (His tag)
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- 抗原 See all LIFR 蛋白
- LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))
- 蛋白类型
- Recombinant
- 产品特性
- AA 45-833
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This LIFR protein is labelled with His tag.
- 原理
- Human LIF R/CD118 Protein
- 序列
- Gln45-Ser833
- 产品特性
- Recombinant Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.75μg/ml determined by ELISA. The affinity constant of 0.662 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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Leukemia Inhibitory Factor Receptor alpha (LIFR) (AA 45-833) protein (Fc Tag)
LIFR 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Leukemia Inhibitory Factor Receptor alpha (LIFR) (AA 45-833) (Active) protein (Fc Tag)LIFR 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Leukemia Inhibitory Factor Receptor alpha (LIFR) (Active) protein (His tag)LIFR 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Leukemia Inhibitory Factor Receptor alpha (LIFR) (Active) protein (His tag)LIFR 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))
- 别名
- LIF R (LIFR 产品)
- 背景
- The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.
- 分子量
- 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
- UniProt
- P42702-1
- 途径
- JAK/STAT Signaling, Growth Factor Binding
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