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IL-25 Protein (AA 33-177) (His tag)

IL25 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7274920
发货至: 中国
  • 抗原 See all IL-25 (IL25) 蛋白
    IL-25 (IL25) (Interleukin 25 (IL25))
    蛋白类型
    Recombinant
    产品特性
    AA 33-177
    宿主
    • 16
    • 10
    • 2
    资源
    • 18
    • 5
    • 1
    • 1
    • 1
    HEK-293 Cells
    标记
    This IL-25 protein is labelled with His tag.
    原理
    Human IL-25/IL-17E Protein
    序列
    Tyr33-Gly177
    产品特性
    Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
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  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    IL-25 (IL25) (Interleukin 25 (IL25))
    别名
    IL-25 (IL25 产品)
    背景
    IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
    分子量
    17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
    UniProt
    Q9H293-1
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