IL-18 Protein (AA 37-193) (Biotin)
Our Local Distributor
北京 101111
Quick Overview for IL-18 Protein (AA 37-193) (Biotin) (ABIN7274881)
抗原
See all IL-18 (IL18) 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
产品特性
- AA 37-193
-
标记
- This IL-18 protein is labelled with Biotin.
-
原理
- Biotinylated Human IL-18 Protein (Primary Amine Labeling)
-
序列
- Tyr37-Asp193
-
产品特性
- Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag., It contains Tyr37-Asp193.
-
过滤
- 0.22 μm filtered
-
内毒素水平
- Less than 1 EU per μg by the LAL method.
-
Biological Activity Comment
- Human IL-18 R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Biotinylated Human IL-18, No Tag with an affinity constant of 3.46 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-18BP, hFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 5.2 ng/ml determined by ELISA.,Biotinylated Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human IL-18 is greater than 90% as determined by SEC-HPLC.
-
-
Interleukin 18 (IL18) (AA 37-193) protein (His-IF2DI Tag)
IL18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 18 (IL18) (AA 1-193) protein (GST tag)IL18 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 18 (IL18) protein (His tag)IL18 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 18 (IL18) protein (GST tag)IL18 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % as determined by reducing SDS-PAGE.
Interleukin 18 (IL18) (AA 36-192) protein (His tag)IL18 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Interleukin 18 (IL18) (AA 37-197) protein (Fc Tag)IL18 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 (IL18) (AA 37-197) protein (mFc Tag)IL18 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 (IL18) (AA 36-192) proteinIL18 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
-
缓冲液
- Lyophilized from 0.22 μm filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
-
储存条件
- -20 °C,-80 °C
-
储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
-
有效期
- 12 months
-
-
- IL-18 (IL18) (Interleukin 18 (IL18))
-
别名
- IL-18
-
背景
- Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
-
分子量
- 18.2 kDa same as Bis-Tris PAGE result.
-
UniProt
- Q14116
-
途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原
-
