GFRAL Protein (AA 20-350) (His-Avi Tag,Biotin)
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- 抗原 See all GFRAL 蛋白
- GFRAL (GDNF Family Receptor alpha Like (GFRAL))
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-350
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This GFRAL protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Mouse GFRAL/GFR alpha-like Protein
- 序列
- Gln20-Glu350
- 产品特性
- Recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu350.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product GFRAL 蛋白
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GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (His-Avi Tag)
GFRAL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 20-350) protein (His-Avi Tag)GFRAL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (His-Avi Tag,Biotin)GFRAL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (Fc Tag)GFRAL 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 20-351) protein (His-Avi Tag)GFRAL 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 20-351) protein (His-Avi Tag,Biotin)GFRAL 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha Like (GFRAL) (AA 19-351) protein (His tag)GFRAL 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- GFRAL (GDNF Family Receptor alpha Like (GFRAL))
- 别名
- GFRAL (GFRAL 产品)
- 背景
- GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
- 分子量
- 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q6SJE0-1
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