FcRn Protein (AA 24-297) (His tag)
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- 抗原 See all FcRn 蛋白
- FcRn (neonatal Fc Receptor (FcRn))
- 蛋白类型
- Recombinant
- 产品特性
- AA 24-297
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宿主
- Cynomolgus, 猕猴
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资源
- HEK-293 Cells
- 标记
- This FcRn protein is labelled with His tag.
- 原理
- Cynomolgus/Rhesus macaque FcRn Protein
- 序列
- Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
- 特异性
- Uni-Prot: Q8SPV9 (FCGRT), Q8SPW0 (B2M)
- 产品特性
- Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala24-Ser297 (FCGRT)&Ile21-Met119 (B2M).
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 0.18 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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neonatal Fc Receptor (FcRn) (AA 24-297) protein (His tag)
FcRn 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
neonatal Fc Receptor (FcRn) (AA 22-297) protein (His tag)FcRn 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
neonatal Fc Receptor (FcRn) (AA 24-297) protein (His-Avi Tag,Biotin)FcRn 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SEC-HPLC, SPR
neonatal Fc Receptor (FcRn) (AA 24-297) protein (His-Avi Tag)FcRn 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % by SEC-HPLC SDS, SEC-HPLC, SPR
neonatal Fc Receptor (FcRn) (AA 51-160) protein (GST tag)FcRn 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
neonatal Fc Receptor (FcRn) (AA 24-297) (Active) protein (His tag,Strep Tag)FcRn 宿主: Cynomolgus, 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
neonatal Fc Receptor (FcRn) (AA 24-297) (Active) protein (His tag,Strep Tag)FcRn 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
neonatal Fc Receptor (FcRn) (AA 22-297) protein (His tag,Strep Tag)FcRn 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
neonatal Fc Receptor (FcRn) (AA 24-297) (Active) protein (His tag,Strep Tag,AVI tag,Biotin)FcRn 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
neonatal Fc Receptor (FcRn) (AA 22-297) protein (His tag)FcRn 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- FcRn (neonatal Fc Receptor (FcRn))
- 别名
- FcRn (FcRn 产品)
- 背景
- The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
- 分子量
- 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result.
- UniProt
- Q8SPV9
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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