EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag)
Quick Overview for EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag) (ABIN7274553)
抗原
See all EPH Receptor B2 (EPHB2) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 19-543
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标记
- This EPH Receptor B2 protein is labelled with His tag.
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原理
- Human EPHB2 Protein
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序列
- Val19-Leu543
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产品特性
- Recombinant Human EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Leu543.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human EPHB2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA. See testing image for detail.
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EPH Receptor B2 (EPHB2) (AA 19-542) protein (His tag)
EPHB2 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor B2 (EPHB2) (AA 19-543) protein (His tag)EPHB2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
EPH Receptor B2 (EPHB2) (AA 570-987) (Active) protein (GST tag,His tag)EPHB2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
EPH Receptor B2 (EPHB2) (AA 19-543) protein (His tag)EPHB2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- EPH Receptor B2 (EPHB2)
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别名
- EPHB2
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背景
- EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.
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分子量
- 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
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UniProt
- P29323-1
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途径
- RTK signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
抗原
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