DLL4 Protein (AA 27-524) (Fc Tag)
Quick Overview for DLL4 Protein (AA 27-524) (Fc Tag) (ABIN7274485)
抗原
See all DLL4 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 27-524
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标记
- This DLL4 protein is labelled with Fc Tag.
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原理
- Human DLL4 Protein
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序列
- Ser27-Pro524
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产品特性
- Recombinant Human DLL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser27-Pro524.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human DLL4, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA. The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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delta-Like 4 (DLL4) (AA 27-524) protein (His-Avi Tag,Biotin)
DLL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
delta-Like 4 (DLL4) (AA 558-685) protein (His-IF2DI Tag)DLL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
delta-Like 4 (DLL4) (Active) protein (His tag)DLL4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- DLL4 (delta-Like 4 (DLL4))
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别名
- DLL4
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背景
- Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
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分子量
- 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Tris-Bis PAGE result.
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UniProt
- Q9NR61
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途径
- Notch Signaling
抗原
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