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CDCP1 Protein (AA 30-666) (His tag)

CDCP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7274293
发货至: 中国
  • 抗原 See all CDCP1 蛋白
    CDCP1 (CUB Domain Containing Protein 1 (CDCP1))
    蛋白类型
    Recombinant
    产品特性
    AA 30-666
    宿主
    • 14
    • 4
    • 2
    • 1
    • 1
    资源
    • 15
    • 3
    • 2
    • 1
    • 1
    HEK-293 Cells
    标记
    This CDCP1 protein is labelled with His tag.
    原理
    Human CDCP1 Protein
    序列
    Phe30-Leu666
    产品特性
    Recombinant Human CDCP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe30-Leu666.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human CDCP1 at 2μg/ml (100μl/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    CDCP1 (CUB Domain Containing Protein 1 (CDCP1))
    别名
    CDCP1 (CDCP1 产品)
    背景
    Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
    分子量
    72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
    UniProt
    Q9H5V8-1
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