CD3E & CD3G (AA 23-120) protein (His tag)
Quick Overview for CD3E & CD3G (AA 23-120) protein (His tag) (ABIN7274144)
抗原
蛋白类型
宿主
资源
纯度
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产品特性
- AA 23-120
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标记
- His tag
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原理
- Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein
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序列
- Asp23-Glu120(CD3E) & Gln23-Asn106(CD3G)
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产品特性
- Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120(CD3E)&Gln23-Asn106(CD3G).
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human CD3E&CD3G, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA. The affinity constant of 0.22nM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD3E & CD3G (AA 23-126) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 22-117) protein (Fc Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 23-126) protein (Fc-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 23-120) protein (His tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 22-117) protein (Fc Tag,Biotin)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 23-108) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD3E & CD3G (AA 23-126) (Active) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3E & CD3G (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
CD3E & CD3G (AA 22-117) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3E & CD3G (AA 23-108) (Active) protein (His tag,DYKDDDDK Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- CD3E & CD3G
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别名
- CD3E&CD3G
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背景
- T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
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分子量
- 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.
抗原
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