Poliovirus Receptor Protein (PVR) (AA 29-348) (His tag)
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Quick Overview for Poliovirus Receptor Protein (PVR) (AA 29-348) (His tag) (ABIN7274094)
抗原
See all Poliovirus Receptor (PVR) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 29-348
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标记
- This Poliovirus Receptor protein is labelled with His tag.
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原理
- Mouse CD155/PVR Protein
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序列
- Asp29-Leu348
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产品特性
- Recombinant Mouse CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp29-Leu348.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 uM as determined in SPR assay (Biacore T200).,Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA (QC Test).,Mouse CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse CD155, His Tag on Anti-His-Biosensor can bind Rat TIGIT, mFc Tag with an affinity constant of 6.93 nM as determined in BLI assay (Gator® Prime).,The purity of Mouse CD155 is greater than 95% as determined by SEC-HPLC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Poliovirus Receptor (PVR)
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别名
- CD155
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背景
- CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
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分子量
- 35.9 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
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UniProt
- Q8K094
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome
抗原
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